ratio (SBR) at 6 different time points (0, 3, 5, 15, 30, and 60 min and compared the performance (n 3 per each fluorophore). Park MH, Hyun H, Ashitate Y, Wada H, Park G, Lee. Inserm, CRI, U823, Institut Albert Bonniot, 38042 Grenoble, France. (Click on the image to enlarge.) (Click on the image to enlarge.) Table 1 Optical properties of NIR fluorophores in 100 serum supplemented with 50 mM hepes (pH.4). All NIR fluorophores except for MB were diluted into 5 dextrose in water (D5W) before injection, and 100 L was injected into mice and 500 L was injected into rats, respectively. Lymph nodes were clearly visualized even when obscured by overlying pancreatic tissue, with the color merged image showing all desired surgical landmarks on one screen. The microscope was equipped with a 100 W mercury light source, NIR-compatible optics, and a NIR-compatible 4X, 10X, 20X, and 40X Plan Fluor objective lens (Nikon, Melville, NY).
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Abbreviations used are: Pa/Mu, pancreas-to-muscle ratio; Pa/Sp, pancreas-to-spleen ratio; Pa/LN, pancreas-to-lymph node ratio; Pa/Ki, pancreas-to-kidney ratio. Pancreas-related complications in abdominal surgery are serious and sometimes fatal. Synthesis and in vivo fate of zwitterionic near-infrared fluorophores. B) Histological analysis: Consecutive sections from rat pancreas stained with H E or unstained were observed using an NIR fluorescence microscope. For large animal swine studies, 164 mol (1.5 mg/kg) of MB and.5 mol of T700-F were injected intravenously.
2013; 10 :507-18. Edu or Maged Henary,. Okabayashi T, Kobayashi M, Sugimoto T, Okamoto K, Matsuura K, Araki. Patient Journey, patient Resources, consultation locations, endoscopy locations, follow. Of note, no adverse reactions were encountered over the 8 h observation period. 2012; 176 :7-13. Following anesthesia, a 14-G central venous catheter was inserted into the external jugular vein, and saline was administered as needed. Image acquisition and analysis was performed using iVision software (BioVision Technologies, Exton, PA). Available from m, objective: Pancreas-related complications are some of the most serious ones in abdominal surgery. To optimize the best imaging time for pancreas (Pa we injected 25 nmol of T700-F intravenously into CD-1 mice and measured the SBR of pancreas and surrounding tissues over the course of.
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Prevention of postoperative pancreatic fistula after total gastrectomy. NIR Fluorescence Imaging System: The dual-NIR channel flare imaging system has been described in detail previously 17,. We further investigated the kinetics of T700-F for up to 8 h by comparing signals in the pancreas (Pa) with surrounding tissue and organs such as muscle (Mu spleen (Sp lymph nodes (Ln and kidneys (Ki) (Figure 4 C). Frangioni is currently CEO of Curadel, LLC, which has licensed flare imaging systems and contrast agents from the Beth Israel Deaconess Medical Center. 2003; 2 :37-49. 3rd row: Pan lymph node imaging with 1 mol intravenous injection of ZW800-3C 4 h prior to imaging. Image-guided surgery using invisible near-infrared light: fundamentals of clinical translation. Statistical analysis between MB and T700-F was performed using unpaired t test.